Infectious Bursal Disease virus strain Lukert ≥104.3 TCID50* per dose.

*TCID50 = 50% tissue culture infective dose

For the active immunisation of broilers and future layers/breeders to reduce clinical signs caused by Infectious Bursal Disease virus.

Coarse spray or in drinking water use.

Administer via drinking water.

One dose to chickens at 7 to 28 days of age. A second vaccination should be administered at 7 to 10 days after the first vaccination.

Administer via spray or drinking water.

One dose to chickens from 1 day of age.

Depending on the level of field virus present, repeat administration of the vaccine may be required at 7 to 10 days after the first vaccination. If not vaccinated at day old, it is recommended to determine the date of first vaccination according to the level of maternally derived antibodies (MDAs).

Nipple-drinking systems are not an appropriate method of vaccine delivery for 1-3 day old chicks.

Upon reconstitution, light peach to a tan-brownish suspension depending on volume of water added.

Fill a container with an appropriate amount of preferably cold, fresh, non-chlorinated water that is free from metal-ions. Chlorine at levels as low as 1 ppm is known to have a detrimental effect on vaccine virus stability, therefore it’s recommended for drinking water application to add low-fat skimmed milk powder (i.e. < 1% fat, 2–4 grams per litre) or skimmed milk (20–40 ml per litre of water) to the water to prolong the life of the virus in the vaccine suspension. Only skimmed milk should be used, as the fat in whole milk may block the automatic drinking systems as well as reduce vaccine virus efficacy. After mixing, the solution should be allowed to stand for 15–30 minutes before adding the vaccine.

Remove the cap of the vaccine vial. Open the vaccine vial under water and dissolve thoroughly in the container. Empty the vaccine vial completely by rinsing the vial and the stopper in the water.

Administer to birds immediately after dilution.

Avoid exposure of the vaccine suspension to sunlight.

Make sure that all conduit pipes, tubing, troughs, drinkers etc are thoroughly clean and free of any trace of disinfectants, detergents, soap, etc. Contact with disinfectants makes the vaccine ineffective.

Allow water to be consumed so that levels in drinkers are minimal before vaccine is applied. All tubing should be emptied of plain water, so that the drinkers only contain vaccine water. It may be necessary to withhold water on some sites prior to vaccination in order to ensure that all birds drink during the vaccination period. Ideally, vaccine should be administered in the volume of water consumed by the birds in up to 3 hours. The aim is to give every bird one dose of vaccine. As a general rule, apply reconstituted vaccine to chlorine-free and fresh water at the rate of 1,000 doses of the vaccine to 1 litre of water per day of age for 1,000 chickens, e.g. 10 litres would be needed for 1,000 10-day old chickens. If in doubt, measure water intake the day before administering vaccine.

This method is for day old broiler chicks only and may be used in the hatchery or on farm.

Use clean vaccination materials only. No disinfectants or other substances impairing the performance of the live vaccine should be used in the sprayer.

Dilute and administer the reconstituted vaccine at a rate of one dose of reconstituted vaccine per bird, according to the directions of your specific coarse spray vaccination equipment. The recommended volume for 1 dose is between 0.1 and 0.5 ml. The spraying distance should be between 30 and 80 cm above the animals in order to ensure an even distribution and the recommended droplet size is greater than 100 µm.

Turn off ventilation during vaccination.

Ensure that birds are neither overheated nor chilled following coarse spray.

Vaccinate healthy animals only.

Do not use in birds in lay and within 4 weeks before the start of the laying period.

No information is available on the safety and efficacy of this vaccine when used with any other veterinary medicinal product, except for the concurrent use with Poulvac IB H120, administered to broilers at one-day of age. A decision to use this vaccine before or after any other veterinary medicinal product, besides Poulvac IB H120, therefore needs to be made on a case by case basis.

Do not mix with any other veterinary medicinal product.

Local and/or general reactions after administration of the vaccine can be observed very rarely. Reactions are usually transient.

Poulvac Bursine 2 produces increasing bursal damage on bird to bird passage and this is accompanied by a degree of immunosuppression.

The frequency of adverse reactions is defined using the following convention:

If you notice any serious effects, even those not already listed above or you think that the medicine has not worked, please inform your veterinary surgeon.

Maternally-derived antibodies (MDA) can interfere with the development of active immunity. Where it is likely that recent field infection or vaccination of the parent flock has stimulated a high antibody titre and consequently a high level of MDA, the timing of the vaccination programme should be planned accordingly.

Very virulent Infectious Bursal Disease virus can breakthrough higher maternal antibody levels than vaccine virus.

Field infection is capable therefore of reaching birds before vaccine has a chance to take. Only use as part of a complete Infectious Bursal Disease control programme involving hygiene, biosecurity, management, stocking density and disinfection, etc. Overwhelming levels of field virus may overcome vaccinal immunity.

The vaccine virus can spread to in contact birds.

Care should be taken to ensure that the vaccine virus does not spread to unvaccinated birds. It is recommended to vaccinate all chickens on a site at the same time. Cleaning and disinfection of the premises is advisable between flocks.

Zero days.

If spray vaccination is applied, the user must at least protect their eyes with safety goggles and nose and mouth with a dust mask. A helmet with filtered air circulation may be used instead. It is recommended to wash and disinfect hands and equipment after vaccinating.

Store and transport refrigerated (2°C to 8°C).

Protect from light.

Shelf-life after reconstitution according to directions: 4 hours.

Keep out of the sight and reach of children.

For animal treatment only.

Pack sizes: 10 x 1000, 2000, 2500, 5000 and 10,000 doses.

Not all pack sizes may be marketed.

Any unused veterinary medicinal product or waste materials derived from such veterinary medicinal product should be disposed of in accordance with local requirements.

Vm 42058/4099