Reconstitute one vial of the freeze-dried fraction (Vanguard DA2Pi) aseptically using the contents of one vial of the liquid fraction (Vanguard CPV-L) as diluent. Shake well and immediately inject the entire contents of the reconstituted vial (1 ml) subcutaneously. Do not use chemically sterilized syringes or needles, as these will interfere with the effectiveness of the vaccine.
Basic Vaccination Scheme
Puppies younger than 10 weeks of age
Two doses of Vanguard 7 at least 14 days apart. The first dose can be given as young as 7 weeks of age. The second dose should not be given until at least 10 weeks of age.
Puppies 10 weeks of age and older
A single dose of Vanguard 7, followed by a single dose of Vanguard Lepto ci at least 14 days later.
Rabies
If protection against rabies is required:
First dose: Vanguard 7 from 10 weeks of age.
Second dose: Vanguard Lepto-ci mixed with Versiguard Rabies at least 14 days later, but not before 12 weeks of age.
To mix both products, Vanguard vaccines should be reconstituted as described above. The reconstituted vial will then be well shaken and then mixed with 1 ml of Versiguard Rabies either in the Versiguard Rabies vial or the syringe. Versiguard Rabies will be well shaken before use. The mixed vaccines will be gently shaken and then administered immediately by subcutaneous injection.
The efficacy of the rabies fraction is proven after a single dose from 12 weeks of age in laboratory studies. However, in field studies 10% of seronegative dogs did not show seroconversion (>0.1 IU/ml) 3–4 weeks after single primary vaccination against rabies. Some animals may also not show titres >0.5 IU/ml after the primary vaccination. Antibody titres drop over the course of the 3-year duration of immunity, although dogs are protected when challenged. In case of travelling to risk areas or outside the EU, veterinary surgeons may wish to give additional rabies vaccinations after 12 weeks of age to ensure that the vaccinated dogs have an antibody titre of ≥0.5 IU/ml, which is generally regarded as sufficiently protective and that they meet the travel test requirements (antibody titres ≥0.5 IU/ml).
Although the efficacy of the rabies fraction has been demonstrated following administration at 12 weeks, at the discretion of the veterinary surgeon, in case of need, dogs younger than 10 weeks can be vaccinated with Vanguard 7 mixed with Versiguard Rabies as the safety of this association has been demonstrated in 7-week-old dogs.
Re-vaccination scheme
A single dose of Vanguard 7 should be given annually.
See Further Information section for more detailed guidance relating to duration of immunity.