An aqueous solution of the product is used in an immersion bath for sedation, immobilisation and anaesthesia of fish, both ornamental and those intended for human consumption.
A number of factors influence the efficacy and safety of the product, including concentration of the drug in water, duration of exposure, previous exposures to the drug, temperature, oxygen content and density of biomass. Because of these variable factors it is strongly recommended that a test of the selected drug concentration and exposure time is conducted with a small group of representative fish before large numbers are medicated. This is especially important when water temperature is at the upper or lower ends of the normal temperature ranges for the species being treated. The product should be dissolved in water of the same composition and characteristics as that to which the fish are accustomed. As the product has good aqueous solubility, it may be added directly to the container. Effects on the fish should be monitored as the product is gradually introduced.
Before anaesthesia or prolonged sedation, fish should be fasted for 12 to 24 hours. During treatment they should be stocked at a density not exceeding 80 g/litre. To minimize damage and loss when medicated for long periods for transport etc. the level of sedation should allow fish to maintain their equilibrium and swimming position. Aeration should be provided unless sedation, or anaesthesia, is of short duration. In anaesthesia loss of reflexes takes place in one to fifteen minutes after immersion, depending upon concentration employed. Narcotised fish should be removed from medicated water and returned to their normal environment as soon as possible, when recovery will take between one and thirty minutes.
The following examples of dose rates and exposure times are based on laboratory and field experience:
Species | Level of Anaesthesia | Concentration | Immersion |
| | mg/L water | time (mins) |
Trout species (7-17°C) | Sedation | 10-30 | Up to 480 |
| Anaesthesia | 30 - 180 | Up to 30 |
Salmon species | Sedation | 7-30 | Up to 240 |
| Anaesthesia | 30-100 | Up to 10 |
Carp species | Sedation | 20-30 | Up to 1440 |
| Anaesthesia | 30-200 | Up to 8 |
Fresh water tropical fish | Sedation | 30-50 | Up to 1440 |