Route of administration:
Intralesional use.
Recommended dosage:
A single intra-lesional injection of 1 ml per animal on one occasion.
Preparation of the suspension for injection:
The veterinary medicinal product must be administered intralesionally by a veterinary surgeon taking special precautions to ensure sterility of the injection process. The veterinary medicinal product should be handled and injected using sterile techniques and in a clean environment.
The veterinary medicinal product is required to be administered immediately after thawing in order to maintain cell viability.
Using appropriate gloves, remove the vial from the freezer/liquid nitrogen and thaw immediately at 25 °C–37 °C, e.g. in a water bath, until the content is completely thawed (approximately 5 minutes).
If any cell clusters are visible after thawing, gently invert the vial until the suspension is clear and colourless.
Remove the cap of the vial and aspirate the suspension into a sterile syringe for injection.
Administer using a needle with a diameter greater than or equal to 22G in order to prevent cell damage.
Administer intralesionally under ultrasound guidance with chemical or physical restraint as needed according to good veterinary practice to facilitate a safe intralesional injection. After insertion of the needle into the tendon or ligament, redirect the needle, if necessary, until the lesion is reached. Slowly inject the suspension. In case of a larger lesion the needle can be slowly retracted during injection to facilitate dispersion of the cells throughout the lesion.