The vaccine is to be administered once at the 18th day of embryonation using in-ovo equipment. The injection volume is 0.05 ml per dose. The vaccine is delivered to the amnion sac or, rarely, into the body of the embryo, causing no decrease in hatchability.

1. Calculate and prepare the required volume of the reconstituted vaccine as follows

· a single vial containing 2000 doses of freeze dried component reconstitute in 100 ml solvent,

· a single vial containing 5000 doses of freeze dried component reconstitute in 250 ml solvent, or two vials, containing 2500 doses of freeze dried component reconstitute in 250 ml solvent.

· a single vial containing 10000 doses of freeze dried component reconstitute in 500 ml solvent, or two vials, containing 5000 doses of freeze dried component reconstitute in 500 ml solvent.

2. Draw up 2 ml of the solvent and transfer into the glass vial containing the lyophilised component.

3. Completely dissolve the vaccine by shaking gently and transfer it into the plastic solvent bottle.

4. Rinse the vial with another 2 ml liquid and transfer the rinsing liquid into the plastic solvent bottle.

5. Repeat this rinsing operation.

The reconstituted vaccine should be used within 2 hours.