The product is administered orally, to fish that have received vaccination with AquaVac ERM immersion vaccine 4-6 months previously. Vaccination is administered to fish of not less than 26g weight in a 10-day feeding program, in which feed pellets treated with vaccine are administered according to the following protocol:

The precise bacterial dose taken up by individual fish cannot be calculated.

The vaccine-coated feed is prepared as follows: The vaccine is kept at room temperature (20°C) for 1 hour before use to allow the vaccine to become less viscous. If any separation occurs, the bottle is shaken vigorously until the separated layers are completely dispersed. The feed pellets are turned in a mixer, e.g. concrete mixer and the vaccine is slowly poured or sprayed directly onto the pellets. If a sprayer is used, it should be set to deliver a coarse spray without risk of aerosol particle generation and the spray container must be completely emptied during the mixing operation. The pellets are mixed for at least 2 minutes after all the vaccine has been added. The prepared feed is kept for 1 hour before feeding to allow the vaccine to impregnate the pellets completely.

The vaccine can be mixed with all or one part of the daily ration of feed. Vaccine treated feed should be used to complete the vaccine feeding regime within 19 days of being mixed with vaccine.

The time taken to achieve full effect of vaccination will depend on water temperature. In fish vaccinated by immersion 4.5 months previous to oral vaccination, vaccine efficacy was demonstrated under field conditions at water temperatures of 10°C, 21 days (210 degree days) after completion of the vaccine feeding protocol and protection was observed for the 3 month duration of the field trial.