Immersion - Vaccination Method

1. Shake bottle well before use.

2. Dilute 1 litre of vaccine with 9 litres of clean hatchery water.

3. Drain and weigh a netful of fish and dip fish in the diluted vaccine for 30 seconds ensuring that fish are totally immersed in the vaccine.

4. After 30 seconds exposure lift net, allow to drain and return fish to holding tank.

5. Repeat until 100 kg of fish have been immersed using 1 litre of undiluted vaccine.

6. Spent vaccine is discarded after use.

7. When convenient, more than 1 litre of vaccine may be diluted in a single bath. In such cases the weight of fish which may be dipped will be 100 kg x the number of litres of vaccine used.

336 degree days are required for the development of full immunity (28 days at a water temperature of 12° C). The time for development of protective immunity will depend on water temperature. A duration of immunity of 78 days has been shown under laboratory conditions. Under field conditions, protection may be expected for at least 6 months.

A booster vaccination, administered 4 months after primary vaccination, may induce a better level of protection.