The minimum size of fish for vaccination is 25g.

The product is administered by intra-peritonal injection at a dose of 0.1 ml per fish. Inject fish, previously anaesthetised with an approved anaesthetic, centrally into the abdomen, 1 – 2 fin lengths in front of the base of the pelvic fins. The needle should point forward at an angle of about 45° and in a 25g fish, penetrate to a depth of approximately 2 - 3mm. Administer using a multi-dose injection applicator with a 6 mm, 22 gauge needle, incorporating a mechanism to prevent flush-back. This applies equally to semi-automatic (hand held) and automatic systems. Vaccine should be used within 5 hours of the time when the bottle cap is broached.

Careful injection technique is important to minimise adverse reactions. Use only sterile needles and syringes for administration.