Shake the bottle well before using.

The product is administered by immersion of the fish for 60 seconds in vaccine diluted 1 in 10 with hatchery water. (9 parts water to 1 part vaccine) This process can be repeated, oxygenating the solution between each use with comparable weights of fish, using the same tank of diluted vaccine until 100 kg of fish have been treated per litre of undiluted vaccine. Thus for fish 20 g in weight, 5000 fish may be vaccinated per litre of vaccine. The temperature of diluted vaccine should not differ from the temperature of the water in the holding tank by more than ± 5o C. The precise bacterial dose taken up by individual fish cannot be calculated.

The vaccine must be administered using a multi-dose injection applicator incorporating a mechanism to prevent flush-back. This applies equally to hand-held and automatic systems. The product is administered by intra-peritoneal injection in the ventral area, just anterior to the pelvic fins. The dose is 0.1 ml per fish.The fish should be anaesthetised prior to vaccination, using an anaesthetic licensed for use on fish.

Vaccine should be used by injection within 5 hours of broaching the stopper. Any unused vaccine should be disposed of after this period. When administering by immersion, dilute the contents immediately after opening the container, and use diluted vaccine immediatiately.