The administration of Isoflurane has been shown to be compatible with the commonly used agents such as acepromazine, opioid analgesics, alpha-2-adrenoreceptor agonists and anticholinergic drugs. Isoflurane is also compatible with barbiturates, ketamine and muscle relaxants in the horse, and with barbiturates and propofol in the dog.

Isoflurane does not decompose in the presence of soda lime, and does not react with metals commonly used in anaesthetic equipment.

Reproduction studies performed on the common laboratory animal species have demonstrated no evidence of foetal malformation or effect on fertility. Equivalent data in domestic species have not been established.