Treat once daily at 50mg Bronopol/litre (1ml product /10 litres incubator water) for

30 minutes, as necessary, commencing 24 hours after fertilisation

Efficacy data indicates that the product must be correctly administered.

The correct amount of product per volume of incubator should be measured and mixed with a small volume of incubator water prior to addition to the incubator. Undiluted product in direct contact may damage the surface of plastic troughs.

Use only the measuring cylinder provided to measure out the correct amount of product. After use, rinse the measuring cylinder carefully and add the rinsings back into the incubator water.

Flow rates to the incubator must be such that a complete exchange of the incubator water is achieved in 30 minutes or less, following the end of the treatment period.

It is essential that the fish eggs be exposed to a homogeneous concentration of Pyceze throughout the treatment period. This can be achieved by ensuring there is continual water movement in the incubator during the treatment period by following the recommendations below for either re-circulation or continuous addition depending on your choice of application. It is important to maintain the flow rate in the incubator during the treatment period to that normally used on the farm to prevent any physical damage to the sensitive eggs.

Use the following equations to calculate the amount of product required, depending on your choice of application. It is recommended that you do not change the configuration of your system. The treatment time may appear longer than 30 minutes – this is to allow time for the product to disperse so that each egg receives the correct dose for the correct time. Treat daily.

Total treatment dose is added to the system over the time it takes for the water to circulate once through the system (Administration Time) to achieve proper mixing. Circulation of the medicated water is then continued for the treatment time of 30 minutes

where:

where:

Add the dose of Pyceze during the calculated Administration Time, and then circulate for 30 minutes. The flow of unmedicated water should then resume.

Treatment is added to the system over the time it takes for the water already in the system to achieve the correct dose level. Addition of the medicated water is then continued for the treatment time of 30 minutes.

where:

where:

Add the dose of Pyceze during the calculated Administration Time, leaving the in-flow water flow unchanged.

A laminated instruction card is provided to illustrate these calculations.

If appropriate to the farm management system, dead eggs should be removed on a regular basis to avoid these becoming a source of further fungal infection.

Treat once daily at 20mg Bronopol/litre (1 ml product/25 litres water) for 30 minutes daily, for up to 14 consecutive days. Use the equation below to calculate the amount of product required.

After 30 minutes treatment time resume the unmedicated water flow to the treatment unit.

Efficacy data indicates that the product must be correctly administered.

Using the 500ml measuring jug (provided with the 5 litre pack) add the required volume of product to a bucket containing a suitable volume of water (e.g., 5, 10 or 20 litres) from the treatment bath. Mix the bucket contents thoroughly and after stopping the water flow add to the treatment bath at several positions, spreading the treatment around the treatment bath as much as possible to aid mixing. After use, rinse the measure and the bucket carefully and add the rinsings back into the treatment bath.

Undiluted product in direct contact may damage the surface of plastic tanks.

Flow rates to the treatment unit must be such that a complete exchange of the water is achieved in 60 minutes or less following the end of the treatment period.

It is recommended that oxygen be diffused into the treatment unit throughout the treatment period to maintain an oxygen level above 7mg/litre and to ensure adequate mixing of the product.

Movement of the fish and oxygen diffusion during treatment will ensure mixing of the product through the treatment water. The treatment unit volume may be reduced to limit the amount of product used. Care should be taken when doing this not to unduly stress the fish by lack of space or oxygen availability.

For treatment of freshwater cages the net should be raised to a depth of 1 to 2 metres depending on the biomass of the fish. The cage to be treated should then be surrounded by an impervious tarpaulin to completely isolate it. Oxygen should be supplied and treatment carried out as above. Once the 30 minutes treatment time is completed the tarpaulin should be removed and the nets lowered to the original depth.

Treat daily for up to 14 consecutive days. It may be appropriate to reduce the frequency of treatment where challenge is less severe, depending on advice from the prescribing veterinary surgeon