Food should be withheld for at least 24 hours and the fish must be anaesthetised prior to injection. Inject 0.2ml intraperitoneally per fish.

To reduce the risk of side effects, it is important to deposit the entire dose in the abdominal cavity. The most frequently used injection needle gauge is 0.7 mm diameter (G22) or 0.6 mm diameter (G23). The injection needle should have appropriate length to penetrate the abdominal wall by 1-2 mm. The entire needle should be inserted into the midline about one, to one and a half pelvic fin lengths anterior to the base of the pelvic fin.