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Dosage and administration
Administer a single dose of 0.05 ml per fish. Fish should not be vaccinated more than once.
Administration route
The vaccine should be administered by intraperitoneal (i.p.) injection into the midline about one fin length anterior to the base of the pelvic fin. To reduce the risk of adverse reactions, it is important to deposit the entire dose in the abdominal cavity. The injection needle used should have appropriate length to penetrate the abdominal wall and 1-2 mm into the abdominal cavity.
It is recommended to starve the fish for a minimum of 48 hours before vaccination.
The fish should be anaesthetised prior to injection.
Let the vaccine slowly reach 15-20°C by keeping it at room temperature.
Ensure a homogenous emulsion prior to use by squeezing and shaking the vaccine bag for approx. 2 minutes.
Only administer the vaccine if it appears as a homogenous, white to cream coloured emulsion.
The vaccine should not be used if the vaccine shows signs of a brownish water phase in the bottom of the container. Contact the distributor for further advice.
The injection devices used for vaccination, i.e. automatic vaccination machines or manual syringes, must be designed and suitable for administration of the recommended dose volume in the target species. The devices must be operated by trained personnel and should be calibrated according to the manufacturers' recommendation prior to use. Special care should be taken to ensure air is removed from the injection equipment (chambers and tubes) prior to vaccination. Regular dose controls are recommended.
The vaccination equipment should be thoroughly cleaned / sterilized before use.