Route of administration:
Intra-lesional use.
Dosage:
A single intra-lesional injection of 1 ml per animal on one occasion.
Preparation of the suspension for injection:
The veterinary medicinal product must be administered intra-lesionally by a licensed veterinarian taking special precautions to ensure sterility of the injection process. The veterinary medicinal product should be handled and injected using sterile techniques and in a clean environment.
The veterinary medicinal product is required to be administered immediately after thawing in order to maintain cell viability.
Using appropriate gloves, remove the vial from the freezer/liquid nitrogen and thaw immediately at 25 °C–37 °C, e.g. in a water bath, until the content is completely thawed (approximately 5 minutes).
If any cell clusters are visible after thawing, gently invert the vial until the suspension is clear and colourless.
Remove the cap of the vial and aspirate the suspension into a sterile syringe for injection.
Administer using a needle with a diameter greater than or equal to 22G in order to prevent cell damage.
Administer intra-lesionally under ultrasound guidance with chemical or physical restraint as needed according to good veterinary practice to facilitate a safe intra-lesional injection. After insertion of the needle into the tendon or ligament, redirect the needle, if necessary, until the lesion is reached. Slowly inject the suspension. In case of a larger lesion the needle can be slowly retracted during injection to facilitate dispersion of the cells throughout the lesion.