The vaccine contains inactivated strains of Escherichia coli expressing the adhesins F4ac, F5, F6, F18ab, F18ac and F41 which cause neonatal enterotoxicosis in piglets, as well as β-enterotoxin from Clostridium perfringens type C. The vaccine is formulated with an oily adjuvant. In sows and gilts, the vaccine induces the specific seroconversion of vaccinated animals; piglets are passively immunised by intake of colostrum containing Escherichia coli adhesin-specific and Clostridium perfringens anti-enterotoxin antibodies.